How do doctors determine if someone is cured of HIV? A new Pitt Public Health test may solve the problem efficiently and inexpensively.

PITTSBURGH, PA (PRWEB) MAY 29, 2017

The quest to develop a cure for HIV has long been plagued by a seemingly simple question: How do doctors determine if someone is cured? The virus has a knack for lying dormant in immune cells at levels undetectable to all but the most expensive and time-consuming tests.

Scientists at the University of Pittsburgh’s Graduate School of Public Health announced today in Nature Medicine that they’ve created a test sensitive enough to detect “hidden” HIV, and yet is faster, less labor-intensive and less expensive than the current “gold standard” test.

The new Pitt test also revealed that the amount of virus lurking dormant in people who appear to be nearly cured of HIV is about 70-fold larger than previous estimates.

“Globally there are substantial efforts to cure people of HIV by finding ways to eradicate this latent reservoir of virus that stubbornly persists in patients, despite our best therapies,” said senior author Phalguni Gupta, Ph.D., professor and vice chair of Pitt Public Health’s Department of Infectious Diseases and Microbiology. “But those efforts aren’t going to progress if we don’t have tests that are sensitive and practical enough to tell doctors if someone is truly cured.”

To date, the best test available to do this is called a “quantitative viral outgrowth assay,” or Q-VOA. This test has many drawbacks: It may provide only a minimal estimate of the size of the latent HIV reservoir; requires a large volume of blood; and is labor-intensive, time-consuming and expensive.

The TZA test produces results in one week compared to the two weeks needed using the Q-VOA, and at a third of the cost. It also requires a much smaller volume of blood and is less labor-intensive.

“Using this test, we demonstrated that asymptomatic patients on antiretroviral therapy carry a much larger HIV reservoir than previous estimates—as much as 70 times what the Q-VOA test was detecting,” said Gupta. “Because these tests have different ways to measure HIV that is capable of replicating, it is likely beneficial to have both available as scientists strive toward a cure.”

Because of its low cell requirement, the TZA also may be useful for quantification of replication-competent HIV-1 in the pediatric population, as well as in the lymph nodes and tissues where the virus persists.

The above advancement in technology explains and confirms that HIV Proviral DNA is a very important tool to determine the latent reservoir virus which is very important to ascertain whether the patient under the treatment is really negative and cured from HIV.

And the above article also explains that the presence of residual plasma viremia ie. HIV RNA viral load count ranging from 1 to 49 copies/mL in some infected individuals receiving ART when undergone for their latent viral reservoirs (HIV proviral DNA Proviral load count) carried more than 70 times the residual plasma RNA viral load.

Here again, the focus is on eliminating HIV Proviral DNA for the permanent cure for HIV. However, we were emphasizing the treatment that completely eliminates the HIV Proviral DNA PCR from the body since 2004, as in the year 2004 first batch of 5 patients had recorded Negative for HIV Proviral DNA PCR Qualitative Test and the same was intimated to medical fraternity, health ministry and debated in Indian Parliament on 25th Aug 2004 (Lok Sabha question No. 4112) to view http://www.hootone.org/discuss-in-loksabha.html

Therefore recent research & development on HIV cure concedes with our line of treatment and testing for HIV.
Thus we would be glad to review your health conditions and analyze your health conditions.

Unavailability of the technology to determine the HIV DNA PCR Proviral load count which is also referred as HIV Proviral DNA PCR Quantitative Test in the your respective country are the very common complaints of the patients undergoing the treatment with HOO-IMM PLUS.

Now with the introduction of DBS (Dried Blood Spot) technology, an easy form of bio-sampling. Blood samples are obtained by pricking the heel or finger, blotted and dried on a filter paper. In DBS testing, dried samples are shipped to an analytical laboratory where the samples are then analyzed for specific analyst’s interest.

Therefore you can now check the HIV Proviral load count in your body by undergoing the HIV Proviral load Quantitative test from HBRL, Mumbai.

It only cost $50 and we will send you the DBS Kit along with your medicines. And you have put drop of your blood on the DBS Kit and send it to us and will undergo the above test for you from HBRL and will provide you with HIV DNA proviral load count report within a week.

Kindly find the procedure for the blood collection through DBS below:-
https://www.youtube.com/watch?v=sOfVgRYYPhc

For more detailed information log on to www.hivunani.com
It is a complete mobile friendly website dedicated for Understanding HIV, symptoms, prevention, testing and treatment information.

Also kindly let us know your feedback with regards to your health conditions, improvements and significant changes in the reports, complaints and suggestions with respect to our New Improved Potency Anti-HIV treatment HOO-IMM PLUS. Also kindly fill up the FOLLOW UP form for your register ID.
For free counseling about your health conditions, you can write email to info@hootone.org